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The casein pellet was then collected, washed and resuspended with cold ultrapure water24,26. After centrifugation, the pellet was resuspended in lysis buffer containing 50mM Tris HCl, pH 7.4, with 4% SDS and periodically vortexed for mixing. Subsequently, the samples were subjected to sonication for 5min at room temperature, followed by incubation in water for 5min at 95C.
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