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The casein pellet was then collected, washed and resuspended with cold ultrapure water24,26. After centrifugation, the pellet was resuspended in lysis buffer containing 50mM Tris HCl, pH 7.4, with 4% SDS and periodically vortexed for mixing. Subsequently, the samples were subjected to sonication for 5min at room temperature, followed by incubation in water for 5min at 95C.
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In the past five years since IPL’s inception, CSK has about 1.5 million (consolidated) fans on Facebook; the New York Yankees have about 5 million. But, the Yankees were established back in 1901 CSK only in 2008. CSK continually tweets and keeps its subscribers and fans engaged by giving away awards and rewards, while the players directly promote the CSK summer sports and casual apparel collection online via YouTube videos (see below) fostering a more direct relationship with fan base..
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The National Weather Service was predicting up to 7 inches of heavy, wet snow in southeastern Connecticut and wind gusts that could hit 50 mph. A coastal flood warning was in effect starting Thursday morning for east facing shores in Massachusetts, with up to a 3 foot surge at high tide in some areas. Central Massachusetts was bracing for 4 to 8 inches of snow, while early predictions were that Boston would get less..